AbstractObjectiveImpression cytology is a noninvasive investigation of the ocular surface. It uses the adhesive features of different filter papers to collect a monolayer of epithelial cells from the cornea and/or conjunctiva. Samples obtained by impression cytology exhibit all characteristics of an ideal cytology specimen. The aim of this study was to test the feasibility of impression cytology and determine the most appropriate filter paper to achieve maximum diagnostic value of the feline eye.Animals StudiedTen healthy cats.ProceduresThe study was conducted in two phases. In the first phase, eight different filter papers (FPs) with various pore sizes were tested: 3.0‐, 1.2‐, 0.8‐, 0.45‐, 0.22‐, 0.05‐ and 0.025‐μm cellulose acetate papers and a 0.4‐μm Biopore membrane (BM). Samples were obtained from the superior bulbar and from the inferior palpebral conjunctiva. In the second phase, three different sampling methods – with and without topical anesthesia, and with topical anesthesia and drying of the conjunctiva – were compared employing theBMencased in the intended BM device (BMD). Samples were evaluated for cellularity and quality of cells.ResultsIn the first phase, samples obtained from the superior bulbar conjunctiva with theBMhad the most sufficient cellularity and quality. In the second phase,BMDwith topical anesthesia and additional drying of the conjunctiva was the most ideal method.ConclusionTheBMDmay prove to be a suitable diagnostic tool for clinicians. Sampling is quick, processing is simple, and a large area of intact cells can be harvested.