Following the increase in wild boar population recorded in urban and peri-urban areas throughout Europe, the present survey aimed to assess the occurrence of zoonotic tick-borne pathogens (TBPs) in wild boars living in southern Italy and in their ticks for evaluating the potential risk of infection for animals and humans. From October to December 2019, a total of 176 ticks collected from 93 wild boars andtheir spleen samples were molecularly screened for selected TBPs. Overall, all the wild boars were infested by ticks (mean intensity, 1.9) with Dermacentor marginatus and Ixodes ricinus being identified in 99.4% and 0.6%, respectively. Out of 93 wild boars, 17 (18.3%) were infested by ticks which scored positive to spotted fever group (SFG) Rickettsia species. Rickettsia slovaca and Rickettsia raoultii were identified in 16 (9%) and 1 (0.6%) D. marginatus, respectively, whereas a single I. ricinus (0.6%) was infected by R. slovaca. A single wild boar (1.1%) tested positive to R. slovaca. All ticks and wild boars scored negative to Babesia/Theileria spp., Ehrlichia spp., Anaplasma spp., Candidatus Neoehrlichia spp., Coxiella burnetii and Borrelia burgdorferi sensu lato complex. Data herein obtained suggest that wild boars are involved in the maintenance of D. marginatus in the environment as in peri-urban areas herein investigated. An integrated management approach is advocated for wild boar population control and in preventing the potential risk of TBPs infection in animals and humans.