AbstractPurposeWe investigated the effects of three different unilateral isoinertial resistance training protocols with eccentric overload on changes in lean mass and muscle function of trained (TL) and contralateral non‐trained (NTL) legs.MethodsPhysically active university students were randomly assigned to one of three training groups or a control group (n = 10/group). Participants in the training groups performed dominant leg isoinertial squat training twice a week for 6 weeks (4 sets of 7 repetitions) using either an electric‐motor device with an eccentric phase velocity of 100% (EM100) or 150% (EM150) of concentric phase velocity or a conventional flywheel device (FW) with the same relative inertial load. Changes in thigh lean mass, unilateral leg‐press one‐repetition maximum (1‐RM), muscle power at 40–80% 1‐RM, and unilateral vertical jump height before and after training were compared between the groups and between TL and NTL.ResultsNo changes in any variable were found for the control group. In TL, all training groups showed similar increases (p < 0.05) in 1‐RM strength (22.4–30.2%), lean tissue mass (2.5–5.8%), muscle power (8.8–21.7%), and vertical jump height (9.1–32.9%). In NTL, 1‐RM strength increased 22.0–27.8% without significant differences between groups; however, increases in lean mass (p < 0.001) were observed for EM150 (3.5%) and FW (3.8%) only. Unilateral vertical jump height (6.0–32.9%) and muscle power (6.8–17.5%) also increased in NTL without significant differences between training groups.ConclusionThe three eccentric‐overload resistance training modalities produced similar neuromuscular changes in both the trained and non‐trained legs, suggesting that strong cross‐education effects were induced by the eccentric‐overload training.