AbstractHypoxia induced by eutrophication has become an important factor threatening the survival of coastal life such as Enhalus acoroides. The purpose of the current study was to explore the effect of hypoxia on photosystem II (PSII) of E. acoroides in the dark. The results showed that long‐term dark hypoxia damages PSII activity of E. acoroides. The lower the oxygen content and the longer the hypoxic duration, the more seriously PSII was damaged and the less light‐independent recovery parts of the damaged PSII. The damage to PSII caused by hypoxia was unrelated to ROS but related to respiration, because the respiration rate decreased with the decrease of oxygen content and PSII activity decreased significantly even at a normal oxygen content after the inhibition of aerobic respiratory pathway. Hypoxia reduced energy fluxes between the antennas and the RCs, and generated many inactive RCs, which significantly reduced the electron transfer efficiency of PSII. Severe hypoxia (2.65 mg L−1 oxygen content) caused chlorophyll degradation. The study demonstrated that hypoxia damages PSII of E. acoroides and inhibits PSII recovery in the dark. We suggested that hypoxia together with other environment stressors would be the key reason for the decline of E. acoroides meadows.