ABSTRACTObjectiveSticky bone, created from concentrated growth factor (CGF) or advanced platelet‐rich fibrin (A‐PRF), contains various growth factors. This study analyzed the release patterns of growth factors from CGF sticky bone (CGF‐SB) and A‐PRF sticky bone (A‐PRF‐SB) to inform their application in bone grafting.MethodsCGF, A‐PRF, CGF‐SB, and A‐PRF‐SB were incubated for 28 days, with supernatant collected on days 1, 7, 14, 21, and 28. Growth factors—platelet‐derived growth factor‐BB (PDGF‐BB), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF)—were quantified using multiplex assays. Transforming growth factor‐β1 (TGF‐β1) and insulin‐like growth factor‐1 (IGF‐1) were measured by ELISA.ResultsCGF and A‐PRF released more than half of their PDGF‐BB, VEGF, and bFGF within day 1, followed by rapid declines. Differently, sticky bone released these growth factors more gradually, surpassing 50% cumulative release by day 7 and sustaining over time. IGF‐1 and TGF‐β1 in all groups reached 50% release by days 7 and 14, respectively. A‐PRF‐SB released significantly more PDGF‐BB, bFGF, and TGF‐β1 than CGF‐SB (p < 0.05).ConclusionsBoth CGF‐SB and A‐PRF‐SB exhibit slower and sustained growth factor release compared to CGF and A‐PRF alone, with A‐PRF‐SB offering superior release profiles, highlighting its enhanced potential for bone grafting applications.