Endogenous viral elements are targeted by RNA silencing pathways in banana
Copyright policy )Endogenous viral elements are targeted by RNA silencing pathways in banana
Summary Endogenous banana streak virus (eBSV) integrants derived from three distinct species, present in Musa balbisiana (B) but not Musa acuminata (A) banana genomes are able to reconstitute functional episomal viruses causing banana streak disease in interspecific triploid AAB banana hybrids but not in the diploid (BB) parent line, which harbours identical eBSV loci. Here, we investigated the regulation of these eBSV. In‐depth characterization of siRNAs, transcripts and methylation derived from eBSV using Illumina and bisulfite sequencing were carried out on eBSV‐free Musa acuminata AAA plants and BB or AAB banana plants with eBSV. eBSV loci produce low‐abundance transcripts covering most of the viral sequence and generate predominantly 24‐nt siRNAs. siRNA accumulation is restricted to duplicated and inverted viral sequences present in eBSV. Both siRNA‐accumulating and nonaccumulating sequences of eBSV in BB plants are heavily methylated in all three CG, CHG and CHH contexts. Our data suggest that eBSVs are controlled at the epigenetic level in BB diploids. This regulation not only prevents their awakening and systemic infection of the plant but is also probably involved in the inherent resistance of the BB plants to mealybug‐transmitted viral infection. These findings are thus of relevance to other plant resources hosting integrated viruses.
banana streak virus, [SDV.SA.STA] Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, virus des végétaux, 993, epigenetic regulation, F30 - Génétique et amélioration des plantes, http://aims.fao.org/aos/agrovoc/c_5962, http://aims.fao.org/aos/agrovoc/c_5985, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Gene Expression Regulation, Plant, [SDV.SA.STA]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture, small RNA, RNA, Small Interfering, http://aims.fao.org/aos/agrovoc/c_3224, http://aims.fao.org/aos/agrovoc/c_4995, Badnavirus, pathologie végétale, http://aims.fao.org/aos/agrovoc/c_4993, H20 - Maladies des plantes, Plant Diseases, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Molecular Biology/Genomics [q-bio.GN], Base Sequence, génome, Musa balbisiana, 941, Musa, DNA Methylation, maladie des plantes, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, banana, http://aims.fao.org/aos/agrovoc/c_5974, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, http://aims.fao.org/aos/agrovoc/c_5956, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, RNA Interference, endogenous viral elements, amélioration des plantes
banana streak virus, [SDV.SA.STA] Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, virus des végétaux, 993, epigenetic regulation, F30 - Génétique et amélioration des plantes, http://aims.fao.org/aos/agrovoc/c_5962, http://aims.fao.org/aos/agrovoc/c_5985, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Gene Expression Regulation, Plant, [SDV.SA.STA]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture, small RNA, RNA, Small Interfering, http://aims.fao.org/aos/agrovoc/c_3224, http://aims.fao.org/aos/agrovoc/c_4995, Badnavirus, pathologie végétale, http://aims.fao.org/aos/agrovoc/c_4993, H20 - Maladies des plantes, Plant Diseases, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Molecular Biology/Genomics [q-bio.GN], Base Sequence, génome, Musa balbisiana, 941, Musa, DNA Methylation, maladie des plantes, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, banana, http://aims.fao.org/aos/agrovoc/c_5974, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, http://aims.fao.org/aos/agrovoc/c_5956, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, RNA Interference, endogenous viral elements, amélioration des plantes
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