AbstractObjectiveSpiteful behaviors are those aimed at inflicting harm on another person while also incurring a cost to the self. Although spite sometimes reflects destructive and socially undesirable behaviors including aggression, the current work sought to examine a potentially socially beneficial aspect of spite: engagement in costly punishment for selfish behavior.MethodFour studies used a costly third‐party punishment task and measured individual differences in spite, narcissism, Machiavellianism, psychopathy, and motivations for engaging in punishment.ResultsTrait spite was positively associated with costly punishment of selfish behavior. That association was independent of other dark personality traits (narcissism, Machiavellianism, psychopathy) and was statistically mediated by a desire for retribution. One of the studies also provided evidence that trait spite was associated with costly punishment of even generous behavior; however, rather than a desire for retribution, that association was mediated by a desire to threaten the person being punished.ConclusionPunishing selfishness and other forms of wrongdoing plays an essential role in cooperative group living. The current work provides new insight into the role spiteful motivations might play in this crucial social behavior.