AbstractBackgroundTiletamine‐zolazepam is a widely used as an alternative chemical immobilization method in non‐human primates, with a safe application pathway and necessary relaxation. In order to determine the electrocardiographic parameters ofSapajus libidinosusafter intramuscular tiletamine‐zolazepam administration at the dose of 5 mg/kg, nine animals were submitted to anesthesia.MethodsThe interpretation of the electrocardiogram determined: heart rate in bpm and heart rhythm, P wave, P‐R interval, R wave, QRS complex, T wave, Q‐T interval, corrected QT interval and electrical axis. The mean HR was 206 ± 32 bpm.ResultsThe majority of the monkeys showed normal sinus rhythm, but one animal showed sinus tachycardia. The most commonly observed electrical axis was between +30° and +90°. Two animals presented bigger alterations in the tracing such as low amplitude QRS and T wave bigger than 1/4 of the R wave.ConclusionsThe administration of tiletamine‐zolazepam was safe and efficacious, with minimal cardiovascular effects.