AbstractBackgroundEstablish baseline values for ophthalmic diagnostic tests inSapajus libidinosus.MethodsOphthalmic diagnostic tests, namely Schirmer tear test 1 (STT‐1), intraocular pressure (IOP), B‐mode ultrasound, culture of the bacterial conjunctival microbiota, and conjunctival exfoliative cytology, were performed in 15S. libidinosus.ResultsMean values found were as follows: 2.50 ± 2.94 mm/min for theSTT‐1; 13.3 ± 3.32 mm Hg for theIOP; 2.47 ± 0.41 mm for the depth of the anterior chamber; 2.86 ± 0.96 mm for the axial length of the lens; 10.97 ± 0.48 mm for the depth of the vitreous chamber; and 16.32 ± 1.24 mm for the axial length of the eyeball. The bacterial genus most frequently found wasStaphylococcusspp. Conjunctival cytology showed intermediate epithelial, squamous superficial epithelial, and keratinized cells.ConclusionsDetermination of baseline values for eye measurements and ophthalmic tests will assist in the diagnosis of eye diseases inS. libidinosusmonkeys.