AbstractAimIn the present study, we compared the transportation of three different instrumentation systems in different levels of standardized artificial curved canals.MethodsSixty epoxy resin blocks containing simulated canals were divided into three groups (n = 20) according to the instrument used for canal preparation: Reciproc (REC), Reciproc Blue (REC Blue), and XP‐endo Shaper (XPS). Pre‐ and post‐instrumentation digital images of each specimen were superimposed by Photoshop software to evaluate the different amount of transportation. The Kruskal‐Wallis and Wilcoxon were the non‐parametric tests applied for the intergroup and intragroup analyses, respectively. The significance level was set at 5%.ResultsThe XPS showed significantly less canal transportation than REC Blue at five levels (P < 0.05) and significantly less canal transportation at seven evaluated levels when compared with the REC (P < 0.05). REC Blue showed significant less canal transportation than REC at four of the eight evaluated levels (P < 0.05). All evaluated instruments showed a significantly higher amount of resin removal toward the inner wall compared to the outer wall of the curvature (P < 0.001).ConclusionAlthough the XPS showed significantly less canal transportation than REC and REC Blue, all systems produced transportation from the original canal anatomy.