AbstractAimThe aim of the present study was to evaluate the genotoxicity of non‐alcoholic mouth rinses on buccal epithelial cells using a micronucleus test.MethodsA total of 105 patients were selected and randomly divided into five groups. Four different mouth rinses and normal saline were given for 2 weeks’ duration, and cytological smears were collected before and after exposure. These smears were subjected to micronucleus (MN) and other nuclear abnormalities (ONA) tests using acridine orange stain, and their frequencies were obtained in 500 buccal epithelial cells. The statistical analysis included mean, χ2‐test, analysis of variance, and post‐hoc analysis by Bonferroni test.ResultsMicronucleated cells (P < .00) and MN (P < .00) were higher in individuals exposed to chlorhexidine (CHX), followed by chlorine dioxide (ClO2), potassium nitrate (KNO3), and sodium fluoride (NaF), amine fluoride (AmF), and normal saline. ONA were greater (P < .00) in individuals exposed to CHX, followed by ClO2, AmF, KNO3, and NaF and normal saline. Overall, the results showed that genotoxic damage was greater in the case of CHX, followed by ClO2, KNO3, and NaF, AmF, and normal saline.ConclusionChronic exposure to mouth rinses can cause genotoxic damage to buccal epithelial cells. Long‐term injudicious and inadvertent use of mouth rinses should be discouraged.