AbstractAn in‐house loop‐mediated isothermal amplification (LAMP) method was established for the detection of Salmonella Typhi and Salmonella Paratyphi A using primers that were designed based on gene coding for putative regulatory protein in S. Typhi (STY4220) and S. Paratyphi A (SSPA3213). This established in‐house LAMP assay gave positive results to all the 14 S. Typhi and 20 S. Paratyphi A isolates used in this study and negative to 20 other Salmonella and 19 non‐Salmonella bacteria. When tested on 60 clinical samples, it gave positive results to 10 clinical samples containing either S. Typhi or S. Paratyphi A and negative to the other 50 samples, which were similar to those results obtained by gold standard culture method. Because of its rapidity and simplicity, this LAMP offers a promising method for the detection of both S. Typhi and S. Paratyphi A for screening purposes at resource‐limited settings.Practical ApplicationsCurrent detection methods for S. Typhi and S. Paratyphi A have several limitations; culture method is time‐consuming and laborious, while polymerase chain reaction‐based methods require expensive equipments and trained personnel which are usually not available in the low‐resource setting. Therefore, LAMP established in this study offers a simple, rapid, sensitive and specific method for the screening of S. Typhi and S. Paratyphi A especially at resource‐limited settings.