
pmid: 3065264
The presence of the free opioid pentapeptide methionine enkephalin (ME) and of ME‐containing peptide(s) was established firmly in decalcified, depulped human teeth by using a combination of methods including RP‐HPLC, radioimmunoassay, radioreceptorassay, trypsin, carboxypeptidase B, fast atom bombardment mass spectrometry, and MS/MS methodology. Positive structural identification of ME was made with mass spectrometry. Those data demonstrate the presence of the preproenkephalinergic A system in the human trigeminal sensory termini.
Radioligand Assay, Enkephalin, Methionine, Radioimmunoassay, Humans, Molar, Third, Tooth, Chromatography, High Pressure Liquid, Mass Spectrometry, Peptide Hydrolases
Radioligand Assay, Enkephalin, Methionine, Radioimmunoassay, Humans, Molar, Third, Tooth, Chromatography, High Pressure Liquid, Mass Spectrometry, Peptide Hydrolases
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