Detection of Grapevine A vitivirus in Tunisian grapevines

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 2001 English Publisher:WileyJournal:EPPO Bulletin, volume 31, pages 509-513 (issn: 0250-8052, eissn: 1365-2338,

Authors: Sami Fattouch; H. Acheche; Mohamed Marrakchi; N. Marzouki; Sonia M'hirsi;

doi: 10.1111/j.1365-2338.2001.tb01035.x

Detection of Grapevine A vitivirus in Tunisian grapevines

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Abstract

Grapevine A vitivirus (GVA), Grapevine B vitivirus (GVB), Grapevine leaf‐roll associated closterovirus 3 (GLRaV3) and Grapevine fanleaf nepovirus (GFLV) were detected by DAS‐ELISA in samples of grapevine showing symptoms of rugose wood from different viticultural areas of Tunisia. After optimization of experimental conditions, PCR and IC‐RT–PCR were found to be more efficient and sensitive than DAS‐ELISA for the detection of GVA.

Related Organizations

Institut national des sciences appliquées
France
National Institute of Applied Science and Technology
Tunisia
Link Campus University
Italy

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