Modulation of vascular endothelial growth factor receptors in melanocytes
Copyright policy )Modulation of vascular endothelial growth factor receptors in melanocytes
Abstract: Vascular endothelial growth factor (VEGF) is constitutively produced by keratinocytes, but has no known epidermal target cell. We now report that normal human melanocytes (Mc) maintained in serum‐free, hormone‐, and growth factor‐supplemented medium lacking phorbol ester and choleragen constitutively express VEGF receptor‐1 (VEGFR‐1), VEGFR‐2, and neuropilin‐1. Furthermore, stimulation of Mc with VEGF165 isoform leads to phosphorylation of VEGFR‐2, the receptor responsible for most of the VEGF‐mediated effects in endothelial cells, suggesting that the receptor is functional. Interestingly, in Mc, VEGFR‐2 expression is induced by ultraviolet irradiation and is downregulated by VEGF and tumor necrosis factor‐α. Prolonged culture (>8 weeks) in the presence of phorbol ester abrogates VEGFR‐2 expression, explaining previous reports that Mc do not express VEGFR‐1 and VEGFR‐2. These data suggest that VEGF may play a role in Mc behavior in skin.
- Boston College United States
- Boston University United States
Vascular Endothelial Growth Factor A, Time Factors, Vascular Endothelial Growth Factor Receptor-1, Tumor Necrosis Factor-alpha, Ultraviolet Rays, Blotting, Western, Immunoblotting, Down-Regulation, Vascular Endothelial Growth Factor Receptor-2, Neuropilin-1, Up-Regulation, Receptors, Vascular Endothelial Growth Factor, Phorbol Esters, Humans, Melanocytes, Endothelium, Vascular, Cells, Cultured, Signal Transduction, Skin
Vascular Endothelial Growth Factor A, Time Factors, Vascular Endothelial Growth Factor Receptor-1, Tumor Necrosis Factor-alpha, Ultraviolet Rays, Blotting, Western, Immunoblotting, Down-Regulation, Vascular Endothelial Growth Factor Receptor-2, Neuropilin-1, Up-Regulation, Receptors, Vascular Endothelial Growth Factor, Phorbol Esters, Humans, Melanocytes, Endothelium, Vascular, Cells, Cultured, Signal Transduction, Skin
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