Malaria is an infectious disease responsible for approximately one million deaths annually. The antimalarial effects of angiotensinIIand its analogs againstPlasmodium gallinaceumandP. falciparumhave recently been reported. To evaluate antiplasmodial activity, we synthesized five angiotensinII‐restricted analogs containing disulfide bridges. To accomplish this, peptides containing two inserted amino acid residues (cysteine) were synthesized by the Fmoc solid‐phase method, purified by liquid chromatography, and characterized by mass spectrometry. Conformational studies were performed by circular dichroism. The results indicated that two of the analogs had higher antiplasmodium activity (92% and 98% activity) than angiotensinII(88% activity), measured by fluorescence microscopy. Results showed that the insertion position must be selected, to preserve the hydrophobic interactions between the non‐polar residues, as this affects antiplasmodial activity. The circular dichroism studies suggested that the active analogs as well as the native angiotensinIIadopt aβ‐turn conformation in different solutions. This approach provided insight for understanding the effects of restricting the ring size and position on the bioactivity of angiotensinIIand provides a new direction for the design of potential chemotherapeutic agents.