AbstractBackgroundHuman costal cartilage remains widely used in the reconstruction of soft tissues, particularly within the field of plastic and orthopaedic surgery. The biologic expense of using autologous human costal cartilage has become superseded by the increasingly common use of irradiated costal cartilage allografts. To date, there has been no histologic investigation of such costal cartilage allografts. This study aims to characterize the histologic variations that exist between different costal cartilage specimens, and to quantify this between specimens in spite of their common anatomical derivation.MethodsTwenty‐five specimens of cadaveric human costal cartilage were obtained from Australian Biotechnologies. Each specimen was irradiated, sectioned and stained with Haematoxylin and Eosin, Masson's trichrome and tetrachrome stains. After being analysed under light microscopy, specimen dimensions, chondrocyte counts and mineral content was quantified and measured.ResultsThe median specimen diameter was 8.20 mm, with an interquartile range (IQR) of 1.59 mm. The median measurement from the superficial to basal chondrocyte layer was 1409.91 μm (IQR = 885.59 μm), and the median measurement from superficial to calcified zone was 4146.26 μm (IQR 1441.83 μm). The median chondrocyte area was 442.74 μm2 (IQR = 2622.72 μm2) with their total chondrocyte count ranging from 289 to 591 chondrocytes per square millimetre. The median percentages of collagen and mineral content were 45.17% and 71.82%, respectively (IQR = 20.48%, 14.75%).ConclusionThese findings emphasize the histologic and biochemical degree of variation that exists between specimens of human cadaveric costal cartilage on a microscopic level. This has the potential to influence the selection of costal cartilage allografts for reconstructive purposes.