AbstractIdentification of the specific biomarkers is of great importance to enrich and expand the gonocytes or spermatogonial stem cells (SSCs) in livestock. The glial cell line‐derived neurotrophic factor (GDNF) family receptor alpha‐1 (GFRα‐1) is a conserved marker of the gonocytes/SSCs in multiple species including rodents, primates and human; however, its expression in bovine gonocytes/SSCs is debated. Recently, we and other teams clearly demonstrated the expression of GFRα‐1 in bovine gonocytes/SSCs. This is useful for bovine gonocytes/SSCs‐related research or application. Nonetheless, new methods still need to be developed to identify the undifferentiated spermatogonial subsets in large livestock and elucidate their spermatogenic potency.