The use of pole-zero method for the analysis of smooth muscle network parameters like the myoplasmic resistance, the junctional resistance, and the junctional capacitance is described. The method determines the pole and zero of the equivalent network describing the smooth muscle cells, using the data set obtained from the impedance versus frequency values for tissue strips. The pole-zero method is compared with the frequency response method and the root locus method for the analysis of similar parameters in four strips from guinea pig taenia coli. The values of smooth muscle parameters obtained from all three methods were comparable. The pole-zero method is, however, computationally more efficient and is more precise than the other two methods. The pole-zero method, in determining the smooth muscle parameters, does not deviate from the proposed network model. Furthermore, the pole-zero method does not require phase information as is the case with the root locus method.