Eukaryotic expression of Porcine BST-2 gene and identification of biological activity of BST-2

null Tinghong Zhang; null Zhenjie Zhang; null Xie Zhao; null Weishan Chang

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https://doi.org/10.1109/rsete....

Article . 2011 . Peer-reviewed

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https://dx.doi.org/10.1109/rse...

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Eukaryotic expression of Porcine BST-2 gene and identification of biological activity of BST-2

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 2011Publisher:IEEEJournal:2011 International Conference on Remote Sensing, Environment and Transportation Engineering

Authors: null Tinghong Zhang; null Zhenjie Zhang; null Xie Zhao; null Weishan Chang;

doi: 10.1109/rsete.2011.5966124

Eukaryotic expression of Porcine BST-2 gene and identification of biological activity of BST-2

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Abstract

BST-2 is a new kind of antivirus protein that is induced by IFN α/β. The BST-2 gene fragment was amplified by RT-PCR from pig kidney cells (PK-15) and subcloned into the pLenti7.3/V5-TOPO vector and the positive clone was filtrated. The positive plasmids were transfected into the HEK293T cells. The expressed protein was analyzed by SDS-PAGE. Vesicular stomatitis virus (VSV)-CEF restrain experiment and Porcine reproductive and respiratory syndrome virus (PRRSV) intervence experiment were carried out. Results showed that the sequence of BST-2 gene amplified by RT-PCR was the same as the sequence in gene map of GenBank and its protein molecular weight was 23 kD, which was the same as the fusion protein V5-BST-2, and the recombinant BST-2 has obvious antiviral activity.

Related Organizations

Shandong Agricultural University
China (People's Republic of)

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