Based on the fact that the primary copper sulfide bioleaching has long leaching time and low leaching rate and has not been used in industry, through selective breeding, domesticated species leaching good using the scanning electron microscopy. The chalcopyrite bioleaching by A. ferrooxidans was studied. Understanding of passivation phenomenon of chalcopyrite surface and the types of passivation layer formed. The mechanism of chalcopyrite reaction and passivation reasons was analyzed. Study on chalcopyrite bioleaching by A. ferrooxidans, Ferrous sulfate Pretreatment and Sulfate Pretreatment, The results show that chalcopyrite surface has Obvious bacteria and a mixture of jarosite flock parcel layer when chalcopyrite bioleaching by A. ferrooxidans. When impregnated iron sulfate and ferrous sulfate, leaching of chalcopyrite does not improve the basic problem of delay, but causing obstruction.