Tetrabromobisphenol A (TBBPA) is widely used throughout the world as flame retardant for electronic equipment or building materials, and is detected in air at the dismantling plant, sewage sludge, sediment or human serum samples. In the present study, we examined the effects of TBBPA on soil polyphenol oxidase, catalase and dehydrogenase activity by indoor simulation method. The results showed that the activities of soil polyphenol oxidase and catalase were enhanced significantly at the range of the selected concentration, while inhibited to the dehydrogenase.EC 10 , EC 50 , and EC 90 of TBBPA on polyphenol oxidase and catalase activities was calculated according to the equation obtained from regression model of dose-effect curve. Realizing the effects of TBBPA on the enzymatic diversity in soil can help us to understand the influence and fate of TBBPA in the environment.