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Microrobotic cell injection

Authors: Yu Sun 0001; Bradley J. Nelson;

Microrobotic cell injection

Abstract

Advances in microbiology demonstrate the need for manipulating individual biological cells, such as for cell injection which includes pronuclei injection and intracytoplasmic injection. Conventionally, cell injection has been conducted manually. In this paper, we present a microrobotic system capable of performing automatic embryo pronuclei DNA injection autonomously and semi-autonomously through a hybrid visual servoing control scheme. After injection, the DNA injected embryos were transferred into a pseudopregnant foster female mouse to reproduce transgenic mice for cancer studies. Experimental results show that the injection success rate was 100%. The system setup, hybrid control scheme and other important issues in this application, such as automatic focusing, are discussed.

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Powered by OpenAIRE graph
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
44
Average
Top 10%
Top 10%
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