The automatic logistics and warehousing handling robot AGV is equipped with non-contact control guide, and use a kind of storage battery as a power source. The computer-controlled automatic guiding AGV is developed on the basis of comprehensive analysis of advantages and disadvantages of various modes of guidance, combining absolute and relative positioning application, ultimately I choose the fusion positioning way of Visual navigation and inertial navigation systems. The visual navigation technology used in this article determine the current AGV car's real time location information through the camera on the AGV robot scan the two-dimensional code, then correct this information directly on the inertial navigation system, and readjust the initial parameters for inertial navigation system in order to effectively guarantee AGV positioning accuracy of robot < ± 20 mm. This article includes theoretical analysis of the feasibility of this approach and specific experimental verification of its reliability, finally through the experimental results shows the positioning methods used to regulate AGV robot localization can control its positioning accuracy within ± 20 mm.