The partial transmit sequence (PTS) technique is an effective method for the peak-to-average power ratio (PAPR) reduction of orthogonal frequency division multiplexing (OFDM) signals. Recently, applying channel estimation to PTS-OFDM with different pilot assignment schemes were proposed for removing the requirement of transmitting the side information (SI) to the receiving ends. This paper proposes a novel fixed end pilot assignment (FEPA) scheme which always assigns pilots at both ends of each subblock in PTS-OFDM, and thus avoids the requirement of linear extrapolation in channel estimation. PTS-OFDM with the proposed method for channel estimation may achieve the same bit error rate performance (BER) as that of PTS-OFDM with perfect SI. Simulation results show that the novel pilot assignment scheme keeps the same performance as the traditional PTS but provides better performance in the PAPR reduction and BER compared to other pilot assignment schemes.