Because of the difficulty in analysis and interpretation of surface electromyography (sEMG), the specific muscle contraction synergy associated with low back pain continues to be debated. Streaming topography is a novel method of continuously visualizing the distribution of sEMG signals during dynamic motion to provide a more comprehensive examination and subsequent insight into the synergy of muscle recruitment pattern. The purpose of this study was to assess the feasibility of streaming topography as a diagnostic tool. Ten healthy subjects were recruited to establish the normal pattern of lumbar muscle activity. An array of surface EMG electrodes was applied to the low back region and recorded during forward bending. The root mean square (RMS) of the sEMG signals were calculated as a function of both position and time to produce streaming topographical videos of the muscle activity in the lumbar region. In addition, a preliminary clinical study was carried out with 3 LBP patients. In normal subjects, RMS streaming topography was consistent, reproducible, and reliable. In clinical observation, the RMS streaming topography of LBP patients was obviously different from that of normal subjects. Some of LBP patients showed an asymmetric distribution during symmetric action. Streaming topography provides a dynamic analysis of lumbar muscle activities and illustrates the synergy of muscle contractions, which may be useful to improve physiotherapy management of LBP.