We incorporate the idea by Akashi et al. which utilize disulfide bonds in forming the porous hydrogel. We further develop a system using γ-PGA as scaffold and disulfide bonds cleavage as the critical point of cell sheet detach system. Cytotoxicity tests showed good biocompatibility of the matrix PVDF membranes. Finally, the cell sheet detachment was successfully performed by adding cysteine after DPSC (Dental Pulp Stem Cell) or PDLSC (Periodontal Ligament Stem Cell) cultured for one week.