The Duck Plague Virus (DPV) is the causative agent of Duck plague, which causes significant economic losses in the commercial duck industry all over the world. Our laboratory had identified and sequenced the DPV UL41 gene. Bioinformatics analysis of DPV UL41 gene were performed by some software and online tools. The results showed that GC content of duck plague virus UL41 gene was 42.42%, which encoded a protein with 498 amino acids in the peptide. The UL41 gene sequence didn't contain a rare codons string with more than two rare codons. The construction of phylogenetic tree suggested DPV had a close evolutionary relationship with the branch of the mardivirus genus, including MeHV-1, GaHV-2 and GaHV-3.