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Robust Segmentation and Tracking of Generic Shapes of Neuro-stem Cells

Authors: Ishwar Kulkarni; Uddipan Mukherjee; Chris Sontag; Brian Cummings; M. Gopi 0001;

Robust Segmentation and Tracking of Generic Shapes of Neuro-stem Cells

Abstract

Given the time lapse images of human Neuro Stem Cells (hNSC) marked by fluorescent proteins, that are obtained from a confocal laser microscope, we present algorithms to identify, segment, track, and estimate statistical parameters of the cells. The structure of these cells are quite complex and irregular, which makes segmentation and tracking even more challenging. We use a novel combination of Difference of Gaussians and a variant of the Watershed algorithm to segment cells accurately. Our tracking algorithm can identify not only the temporal path of the cells but also events like cell divisions and deaths. Our system is robust, efficient, completely automatic, and removes many drawbacks faced by earlier solutions. We also propose the first geometric algorithm that uses Delaunay triangulation, to find the number of the branches of the cells, which is an important biological feature.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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