Geographic Information System (GIS) is the miniature of resources and environment of the whole or part region in the computer. It has been used widely in many fields of spatial information systems. There are some development ways to realize GIS. For example, applying MapX controls to realize the basic functions of geographic information system, which is a main way of secondary development application of GIS integration. In this paper, the development and realization of personnel positioning system is mainly based on GIS. In the process of developing the personnel positioning software, it is very important to choose the right development tools. And this positioning system is developed through use of ActiveX technology and LabVIEW programming platform. Their advantages are easy to realize all kinds of personnel positioning functions and map display. The purpose of this paper is to introduce application of GIS in the personnel positioning software. This paper firstly discusses the GIS-related development and then describes the methods of calling the MapX controls. In addition, this paper expounds the realization process of secondary development methods of GIS integration in LabVIEW. Finally, analyzes the running result of personnel positioning software by processing longitude-latitude information that collected by GPS terminal. According to it, the personal positioning information can be also obtained, which is especially useful for monitor of Kids and old people.