This study was designed to evaluate the eye movement parameter of visual fatigue induced during the VDT work, using the sensors to simultaneously record focal eye movement. Inside different time and tasks, the changes in these physiological measures, taken during different VDT tasks, were used to propose an index for evaluating visual fatigue. Test under different experimental conditions takes time as the most important factor observe the changes of eyelid opening, blinking frequency and pupil diameter. These eye movement parameters show significant difference (p<0.05) between the two measurement times. On the basis of the results, the correlation of the eyelid diameter and of the pupil diameter used for evaluation of the visual fatigue was discussed. As a result, there exists the positive relation between pupil diameter and the eyelid opening after long working hours.