Stratified rock mass has been widely existing in geotechnical engineering, especially in tunnel engineering. When tunnel excavated in stratified rock mass, the stress and deformation characteristic of the surrounding rock mass are very different from that in the normal rock mass. In order to study the stress and deformation response tunnel excavation, numerical model is built by the commercial package ANASYS, then the model is transferred based on the self-compiled program to the to the FLAC3D for calculation, then the excavation of tunnel is simulated to find out the effect of the excavation disturbance on the tunnel surrounding rock mass. During simulation procedure, different insitu-stress is set to the model to obtain effect of different horizontal stress on the calculation results, such as the unbalance force of the tunnel surrounding rock mass, the maximum displacement of the tunnel, the horizontal displacement contour and the vertical displacement contour. The calculation result can give some guidance for the real practice.