In image sensor based VLC, transmitter acquisition and tracking are critical issue. However, the fluctuation of the VLC transmitter in the image plane caused by vehicle movement, complicates correct data reception. Therefore, in this paper, we present results of channel fluctuation measurements for infrastructure-to-vehicle VLC (I2V-VLC), vehicle-to-infrastructure VLC (V2I-VLC), and vehicle-to-vehicle VLC (V2V-VLC). We analyze channel fluctuation in terms of optical flow from measured data.