Structure of the X (ADRP) domain of nsp3 from feline coronavirus
Copyright policy )Structure of the X (ADRP) domain of nsp3 from feline coronavirus
The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 A resolution, respectively. In the tetragonal crystal form, glycerol-3-phosphate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 A resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.
- Centre national de la recherche scientifique France
- European Molecular Biology Laboratory Germany
- Leiden University Medical Center Netherlands
- European Bioinformatics Institute United Kingdom
- Deutsches Elektronen-Synchrotron DESY Germany
Molecular Sequence Data, Viral Nonstructural Proteins, Crystallography, X-Ray, alpha-glycerophosphoric acid, Glycerophosphates: metabolism, Protein Interaction Domains and Motifs, Amino Acid Sequence, Coronavirus, Feline, info:eu-repo/classification/ddc/570, Coronavirus, Feline: enzymology, Adenosine Diphosphate Ribose, Binding Sites, Viral Nonstructural Proteins: chemistry, Sequence Homology, Amino Acid, Glycerophosphates: chemistry, RNA-Dependent RNA Polymerase, Adenosine Diphosphate Ribose: chemistry, RNA Replicase, RNA Replicase: chemistry, Glycerophosphates, Adenosine Diphosphate Ribose: metabolism, Sequence Alignment, Protein Binding
Molecular Sequence Data, Viral Nonstructural Proteins, Crystallography, X-Ray, alpha-glycerophosphoric acid, Glycerophosphates: metabolism, Protein Interaction Domains and Motifs, Amino Acid Sequence, Coronavirus, Feline, info:eu-repo/classification/ddc/570, Coronavirus, Feline: enzymology, Adenosine Diphosphate Ribose, Binding Sites, Viral Nonstructural Proteins: chemistry, Sequence Homology, Amino Acid, Glycerophosphates: chemistry, RNA-Dependent RNA Polymerase, Adenosine Diphosphate Ribose: chemistry, RNA Replicase, RNA Replicase: chemistry, Glycerophosphates, Adenosine Diphosphate Ribose: metabolism, Sequence Alignment, Protein Binding
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