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Application of the molecular replacement method to multidomain proteins. 1. Determination of the orientation of an immunoglobulin Fab fragment

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1988Publisher:International Union of Crystallography (IUCr)Journal:Acta Crystallographica Section A Foundations of Crystallography, volume 44, pages 38-45 (issn: 0108-7673,

Authors: W. F. Anderson; Miroslaw Cygler;

doi: 10.1107/s0108767387008365

pmid: 3272145

Application of the molecular replacement method to multidomain proteins. 1. Determination of the orientation of an immunoglobulin Fab fragment

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Abstract

Multidomain proteins provide special problems in the application of the molecular replacement method of structure determination. The structure of the Fab fragment from the autoimmune poly(dT)-specific antibody HED10 has been determined using molecular replacement. An analysis of the effects of varying the model and the parameters used in the rotation function indicates that dividing the molecule into individual relatively rigid domains simplifies interpretation of the results, and that the optimal parameters depend on the molecule under study.

Related Organizations

University of Alberta
Canada

Keywords

Immunoglobulin Fab Fragments, Chemical Phenomena, Models, Chemical, Molecular Structure, Poly T, Chemistry, Physical, Antibodies

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