There is considerable contradiction in the literature concerning the nature and number of radioactive metabolites which can be isolated from bean plants following exposure to C14-labeled 2,4-dichlorophenoxyacetic acid (2,4-D). Thus the isolation of one principal product which was water-soluble and etherinsoluble has been reported (8, 9,10), while on the other hand, Weintraub et al. (17) have suggested that there is an extensive metabolism of the side-chain of 2,4-D, yielding a multiplicity of products. The acid hydrolysis of the water-soluble material gives rise to a radioactive, ether-soluble product, which has been variously identified as 2,4-D (9) or as a substance different from 2,4-D and possibly 6-hydroxy2,4-dichlorophen'oxyacetic acid (6-OH-2 4-D) (8) suggesting that metabolism of the ring occurs. Weintraub (15) has presented evidence that, in microorganisms, one of the chlorine atoms of the 2,4-D is lost. The report by Butts and Fang (6) of the presence of 12 amino acids in the hydrolysis product of the ether-insoluble material suggests the formation of amino acid conjugates of the metabolites of 2,4-D as well. In connection with a study of the induction of callus growth in bean stem sections by 2,4-D (2, 3)? several metabolites of this growth substance were found. The isolation and attempted characterization of these metabolites is presented in this paper. A preliminary report has been published elsewhere (4).