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pmid: 28707908
Recently the physical characterization of a number of biological processes has proven indispensable for a full understanding of natural phenomena. One such example is the mechanical properties of transcription, which have been shown to have significant effects in gene expression. In this letter we introduce a simple description of the basic physical elements of transcription where RNA elongation, RNA polymerase rotation and DNA supercoiling are coupled. The resulting framework describes the relative amount of RNA polymerase rotation and DNA supercoiling that occurs during RNA elongation. Asymptotic behavior is derived and can be used to experimentally extract unknown mechanical parameters of transcription. Incorporation of mechanical limits to RNA polymerase is accomplished yielding an equation of motion for DNA supercoiling and RNA elongation with transcriptional stalling. Important implications for gene expression, chromatin structure and genome organization are discussed.
Quantitative Biology - Subcellular Processes, Transcription, Genetic, DNA, Superhelical, FOS: Biological sciences, Gene Expression, Nucleic Acid Conformation, DNA, DNA-Directed RNA Polymerases, Subcellular Processes (q-bio.SC), Chromatin
Quantitative Biology - Subcellular Processes, Transcription, Genetic, DNA, Superhelical, FOS: Biological sciences, Gene Expression, Nucleic Acid Conformation, DNA, DNA-Directed RNA Polymerases, Subcellular Processes (q-bio.SC), Chromatin
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 23 | |
popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network. | Top 10% | |
influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Average | |
impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Top 10% |