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Physical Review Applied
Article . 2020 . Peer-reviewed
License: APS Licenses for Journal Article Re-use
Data sources: Crossref
https://dx.doi.org/10.48550/ar...
Article . 2019
License: arXiv Non-Exclusive Distribution
Data sources: Datacite
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Snapshot Projection Optical Tomography

Authors: Yongjin Sung;

Snapshot Projection Optical Tomography

Abstract

We present a plenoptic microscopy configuration for 3D snapshot imaging, which is dual telecentric and can directly record true projection images corresponding with different viewing angles. It also allows blocking high-angle stray rays without sacrificing the light collection efficiency. This configuration named as snapshot projection optical tomography (SPOT) arranges an objective lens and a microlens array (MLA) in a 4-f telecentric configuration and places an aperture stop at the back focal plane of a relay lens. We develop a forward imaging model for SPOT, which can also be applied to existing light field microscopy techniques using an MLA as tube lens. Using the developed system, we demonstrate snapshot 3D imaging of various fluorescent beads and a biological cell, which confirms the capability of SPOT for imaging specimens with an extended fluorophore distribution as well as isolated fluorochromes. The transverse and vertical resolutions are measured to be 0.8 $μ$m and 1.6 $μ$m, respectively.

Keywords

Biological Physics (physics.bio-ph), Image and Video Processing (eess.IV), FOS: Electrical engineering, electronic engineering, information engineering, FOS: Physical sciences, Physics - Biological Physics, Electrical Engineering and Systems Science - Image and Video Processing, Physics - Optics, Optics (physics.optics)

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    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    16
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 10%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
16
Top 10%
Top 10%
Top 10%
Green
bronze