Live Cell Imaging in Fission Yeast

descriptionPublicationkeyboard_double_arrow_right Article 21 Jul 2017 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,017, page pdb.top090621 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Mulvihill, Daniel P.;

doi: 10.1101/pdb.top090621

pmid: 28733414

Live Cell Imaging in Fission Yeast

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Abstract

Live cell imaging complements the array of biochemical and molecular genetic approaches to provide a comprehensive insight into functional dependencies and molecular interactions in fission yeast. Fluorescent proteins and vital dyes reveal dynamic changes in the spatial distribution of organelles and the proteome and how each alters in response to changes in environmental and genetic composition. This introduction discusses key issues and basic image analysis for live cell imaging of fission yeast.

Related Organizations

University of Kent
United Kingdom

Keywords

Intravital Microscopy, Staining and Labeling, Schizosaccharomyces, Image Processing, Computer-Assisted, QH426, QH581.2, QR

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