Microinjecting Cells Using a Pulsed-Flow Microinjection System

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2011 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,011, page prot5589 (issn: 1940-3402, eissn: 1559-6095,

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Authors: David A, Dean; Joshua Z, Gasiorowski;

doi: 10.1101/pdb.prot5589

pmid: 21363953

Microinjecting Cells Using a Pulsed-Flow Microinjection System

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Abstract

INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology, and direct delivery of genetic material by microinjection remains a reliable means of transfection. A pulsed-flow microinjection system, such as the Eppendorf InjectMan and FemtoJet systems, permits a high level of control over the microinjection parameters, ensuring reproducibility. The system is also easy to use, is rapid, and is less likely to damage cells than the constant-flow method.

Keywords

Microinjections, DNA, Transfection

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