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Microinjection of Xenopus Oocytes

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 2010Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,010, page pdb.prot5536 (issn: 1559-6095, eissn: 1559-6095,

Authors: Richard M. Harland; Robert M. Grainger; Hazel Sive;

doi: 10.1101/pdb.prot5536

pmid: 21123423

Microinjection of Xenopus Oocytes

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Abstract

INTRODUCTIONXenopus oocytes can be injected with messenger RNA (mRNA) or DNA constructs into the cytoplasm or nucleus, respectively. The cytoplasm can withstand the introduction of up to 50 nL of injected material, and the nucleus can tolerate up to 20 nL. This protocol describes microinjection of both defolliculated and folliculated Xenopus oocytes. Oocytes remain in good condition for a relatively long time, and injections can be continued, if necessary, over a period of days.

Keywords

Cell Nucleus, Cytoplasm, Microinjections, Xenopus, Oocytes, Animals, DNA, RNA, Messenger

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	27
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%