Ant Ecdysteroid Extraction and Radioimmunoassay

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2009 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,009, page pdb.prot5247 (issn: 1559-6095, eissn: 1559-6095,

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Authors: Colin, Brent; Adam, Dolezal;

doi: 10.1101/pdb.prot5247

pmid: 20147212

Ant Ecdysteroid Extraction and Radioimmunoassay

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Abstract

INTRODUCTIONEcdysteroids are a group of steroid compounds present in many plant and invertebrate species. In arthropods, they function primarily as hormones involved in the regulation of molting. This protocol describes how to extract ecdysteroid hormones from ant specimens and subsequently quantify circulating levels of the hormone. The hormone can be extracted from hemolymph or from whole-body homogenates of insects and quantified by radioimmunoassay.

Related Organizations

United States Department of Agriculture
United States
Arizona State University
United States

Keywords

Ants, Hemolymph, Radioimmunoassay, Animals, Ecdysteroids, Biology, Antibodies

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