In Situ Hybridization of Helobdella (Leech) Embryos

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 2009 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,009, page pdb.prot5194 (issn: 1559-6095, eissn: 1559-6095,

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Authors: David A, Weisblat; Dian-Han, Kuo;

doi: 10.1101/pdb.prot5194

pmid: 20147137

In Situ Hybridization of Helobdella (Leech) Embryos

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Abstract

INTRODUCTIONThe purpose of this protocol is to reveal the localization of transcripts in the Helobdella (leech) embryo. In situ hybridization protocols for Helobdella embryos are derived from those used for zebrafish and Xenopus. The protocols are different for early- and late-stage embryos (stages 1-8 and stages 9-11, respectively) due to a difference in tissue permeability.

Related Organizations

University of California, Berkeley
United States

Keywords

Embryo, Nonmammalian, Time Factors, Affinity Labels, Permeability, Genetic Techniques, Leeches, Animals, Oligonucleotide Probes, Vitellins, In Situ Hybridization, Developmental Biology

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