Choline Acetyltransferase Staining in Brain Sections

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 2010 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,010, page pdb.prot4807 (issn: 1559-6095, eissn: 1559-6095,

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Authors: Carol Ann, Paul; Barbara, Beltz; Joanne, Berger-Sweeney;

doi: 10.1101/pdb.prot4807

pmid: 20679372

Choline Acetyltransferase Staining in Brain Sections

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Abstract

INTRODUCTIONThis protocol describes the use of immunocytochemical methods to stain for choline acetyltransferase (ChAT). The avidin-biotin horseradish peroxidase complex (ABC) triple-labeling method used here takes advantage of the extraordinary affinity between avidin and biotin. Horseradish peroxidase attached to biotin in the complex is developed using diaminobenzidine (DAB).

Keywords

Tissue Fixation, Staining and Labeling, Animals, Brain, Choline O-Acetyltransferase, Rats

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