Dejellying Xenopus laevis Embryos

descriptionPublicationkeyboard_double_arrow_right Article 01 May 2007 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,007, page pdb.prot4731 (issn: 1559-6095, eissn: 1559-6095,

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Authors: Hazel L, Sive; Robert M, Grainger; Richard M, Harland;

doi: 10.1101/pdb.prot4731

pmid: 21357076

Dejellying Xenopus laevis Embryos

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Abstract

INTRODUCTIONThis protocol presents a method for preparing Xenopus embryos for manipulation. Embryos are surrounded by a series of thick, protective jelly membranes. Removal of these membranes is the first step in most micromanipulation procedures. These membranes must be completely removed for embryo dissection. For microinjection, membranes can be completely or partially removed.

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