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Microinjection of Protein Samples

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2007Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,007, page pdb.prot4657 (issn: 1559-6095, eissn: 1559-6095,

Authors: Yulia Komarova; John Peloquin; Gary G. Borisy;

doi: 10.1101/pdb.prot4657

pmid: 21356928

Microinjection of Protein Samples

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Abstract

INTRODUCTIONThis protocol describes a method for microinjecting proteins into the nucleus or cytoplasm of adherent cells. Microinjection equipment can be obtained from a number of suppliers; this protocol has been used with the Narishige IM-200 air pressure regulator and the Leitz micromanipulator. Using this system, it is possible to microinject a constant volume within a 50% difference among cells.

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