Plating Cells for Microinjection

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 2006 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,006, page pdb.prot4650 (issn: 1559-6095, eissn: 1559-6095,

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Authors: David A, Dean;

doi: 10.1101/pdb.prot4650

pmid: 22484673

Plating Cells for Microinjection

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Abstract

INTRODUCTIONThis protocol describes a method for plating cells for microinjection onto etched coverslips. The coverslips for microinjection must be marked so that microinjected cells can be identified at time points after injection. A procedure for etching coverslips is given below; alternatively, pre-etched coverslips can be purchased at slightly higher cost.

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