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Protein Arrays: Labeling the Protein and Probing the Array for Protein-Protein Interactions

Name: Protein Arrays: Labeling the Protein and Probing the Array for Protein-Protein Interactions
Creator: Gavin MacBeath
Keywords: 0301 basic medicine, 0303 health sciences, 03 medical and health sciences, 3. Good health

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2007Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,007, page pdb.prot4630 (issn: 1559-6095, eissn: 1559-6095,

Authors: Gavin MacBeath;

doi: 10.1101/pdb.prot4630

pmid: 21357032

Protein Arrays: Labeling the Protein and Probing the Array for Protein-Protein Interactions

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Abstract

INTRODUCTIONTo detect protein-protein interactions, a protein microarray can be incubated with a fluorescently labeled protein. After the array is washed, stable interactions can be identified by scanning the slide for fluorescent spots. This protocol uses cyanine-3 (Cy3) or cyanine-5 (Cy5) monofunctionally activated dyes to label the probe proteins.

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