Standardized rearing methods for the yellow fever mosquitoAedes aegyptiare critical to facilitate controlled laboratory studies. This protocol describes a batch rearing protocol forAedes aegyptistocks that yields healthy eggs, larvae, pupae, and adults in the laboratory for long-term colony maintenance and experimental manipulation. Foundational principles for the rearing and containment of these life cycle stages, as well as steps for mating and blood feedingAedes aegyptito yield viable eggs for continuous culture or storage, are detailed.