Dephosphorylation of Plasmid DNA

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 2020 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,020, page pdb.prot101253 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Michael R, Green; Joseph, Sambrook;

doi: 10.1101/pdb.prot101253

pmid: 33139502

Dephosphorylation of Plasmid DNA

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Abstract

This protocol presents a method to remove 5′-phosphate residues from protruding or blunt termini of linearized plasmids. This suppresses the recircularization of plasmid DNA during subsequent ligation procedures.

Keywords

Transformation, Genetic, Escherichia coli, DNA, Cloning, Molecular, Alkaline Phosphatase, Phosphates, Plasmids

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Fields of Science (3) View all

medical and health sciences

basic medicine

Fields of Science

medical and health sciences

basic medicine

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